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PCR (Polymerase Chain Reaction) (cont.)

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What is the purpose of doing a PCR (polymerase chain reaction)?

To do PCR, the original DNA that one wishes to copy need not be pure or abundant. It can be pure but it also can be a minute part of a mixture of materials. So, PCR has found widespread and innumerable uses -- to diagnose genetic diseases, do DNA fingerprinting, find bacteria and viruses, study human evolution, clone the DNA of an Egyptian mummy, establish paternity or biological relationships, etc.. Accordingly, PCR has become an essential tool for biologists, DNA forensics labs, and many other laboratories that study genetic material.

How was PCR (polymerase chain reaction) discovered?

PCR was invented by Kary Mullis. At the time he thought up PCR in 1983, Mullis was working in Emeryville, California for Cetus, one of the first biotechnology companies. There, he was charged with making short chains of DNA for other scientists. Mullis has written that he conceived of PCR while cruising along the Pacific Coast Highway 128 one night on his motorcycle. He was playing in his mind with a new way of analyzing changes (mutations) in DNA when he realized that he had instead invented a method of amplifying any DNA region. Mullis has said that before his motorcycle trip was over, he was already savoring the prospects of a Nobel Prize. He shared the Nobel Prize in chemistry with Michael Smith in 1993.

As Mullis has written in the Scientific American: "Beginning with a single molecule of the genetic material DNA, the PCR can generate 100 billion similar molecules in an afternoon. The reaction is easy to execute. It requires no more than a test tube, a few simple reagents, and a source of heat."

What is RT PCR?

RT-PCR (Reverse transcriptase-polymerase chain reaction) is a highly sensitive technique for the detection and quantitation of mRNA (messenger RNA). The technique consists of two parts:

  • The synthesis of cDNA (complementary DNA) from RNA by reverse transcription (RT) and
  • The amplification of a specific cDNA by the polymerase chain reaction (PCR).

RT-PCR has been used to measure viral load with HIV and may also be used with other RNA viruses such as measles and mumps.

Previous contributing author and editor:
Medical Author: Frederick Hecht, MD, FAAP, FACMG
Medical Editor: Leslie J. Schoenfield, M.D., Ph.D.

Medically reviewed by Rambod Rouhbakhsh, MD, MBA, FAAFP; American Board of Family Medicine

REFERENCE:

"Tools for genetics and genomics: Polymerase chain reaction"
uptodate.com


Medically Reviewed by a Doctor on 2/4/2014

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Source: MedicineNet.com
http://www.medicinenet.com/pcr_polymerase_chain_reaction/article.htm

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