Definition of cDNA

cDNA: Complementary DNA. cDNA is single-stranded DNA made in the laboratory from a messenger RNA template under the aegis of the enzyme reverse transcriptase. This form of DNA is often used as a probe in the physical mapping of a chromosome.

A cDNA library is a collection of DNA sequences generated from mRNA sequences. This type of library contains only DNA that codes for proteins and does not include any non-coding DNA.

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