Antinuclear Antibody Test (ANA)
William C. Shiel Jr., MD, FACP, FACR
Dr. Shiel received a Bachelor of Science degree with honors from the University of Notre Dame. There he was involved in research in radiation biology and received the Huisking Scholarship. After graduating from St. Louis University School of Medicine, he completed his Internal Medicine residency and Rheumatology fellowship at the University of California, Irvine. He is board-certified in Internal Medicine and Rheumatology.
Charles Patrick Davis, MD, PhD
Dr. Charles "Pat" Davis, MD, PhD, is a board certified Emergency Medicine doctor who currently practices as a consultant and staff member for hospitals. He has a PhD in Microbiology (UT at Austin), and the MD (Univ. Texas Medical Branch, Galveston). He is a Clinical Professor (retired) in the Division of Emergency Medicine, UT Health Science Center at San Antonio, and has been the Chief of Emergency Medicine at UT Medical Branch and at UTHSCSA with over 250 publications.
- What are antinuclear antibodies?
- How is the ANA test designed? How is the procedure performed?
- What is the interpretation of the ANA test result?
- What are autoimmune diseases?
- What other conditions cause ANAs to be produced?
- Can medications cause ANAs to be produced?
- ANAs are defined as having patterns. What does this mean?
- Are ANAs always associated with illness? What is the normal range?
What are antinuclear antibodies?
We normally have antibodies in our blood that repel invaders in our bodies, such as virus and bacteria microbes. Antinuclear antibodies (ANAs) are unusual antibodies, detectable in the blood, that have the capability of binding to certain structures within the nucleus of the cells. The nucleus is the innermost core within the body's cells and contains the DNA, the primary genetic material. ANAs are found in patients whose immune system may be predisposed to cause inflammation against their own body tissues. Antibodies that are directed against one's own tissues are referred to as autoantibodies. The propensity for the immune system to work against its own body is referred to as autoimmunity. ANAs suggest the possible presence of autoimmunity. Therefore, when they are detected in a patient's blood (referred to as a "positive" result), doctors will consider the possibility that an autoimmune illness exists in that patient.
How is the ANA test designed? How is the procedure performed?
The ANA test was designed by Dr. George Friou in 1957. The ANA test is performed using a blood sample. An ANA test is performed by testing the blood in the laboratory. The antibodies in the serum of the blood are exposed in the laboratory to cells. It is then determined whether or not antibodies are present that react to various parts of the nucleus of cells. Thus, the term anti-"nuclear" antibody. Fluorescence techniques are frequently used to actually detect the antibodies in the cells, thus ANA testing is sometimes referred to as fluorescent antinuclear antibody test (FANA).
What is the interpretation of the ANA test result?
The ANA test is a sensitive screening test used to detect autoimmune diseases. Autoimmune diseases feature a misdirected immune system, and each of them has characteristic clinical manifestations that are used to make the precise diagnosis. The interpretation or identification of a positive ANA test does not make a diagnosis. It simply suggests to the doctor to consider the possibility that an autoimmune disease is present.
What are autoimmune diseases?
Autoimmune diseases are conditions in which there is a disorder of the immune system characterized by the abnormal production of antibodies (autoantibodies) directed against the tissues of the body. Autoimmune diseases typically feature inflammation of various tissues of the body. Frequently, ANAs are found in patients with a number of different autoimmune diseases, such as systemic lupus erythematosus, Sjögren's syndrome, rheumatoid arthritis, polymyositis, scleroderma, Hashimoto's thyroiditis, juvenile diabetes mellitus, Addison disease, vitiligo, pernicious anemia, glomerulonephritis, and pulmonary fibrosis. ANAs can also be found in patients with conditions that are not considered classic autoimmune diseases, such as chronic infections and cancer.
Find out what women really need.