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Definition of EST (expressed sequence tag)

  • Medical Author:
    William C. Shiel Jr., MD, FACP, FACR

    Dr. Shiel received a Bachelor of Science degree with honors from the University of Notre Dame. There he was involved in research in radiation biology and received the Huisking Scholarship. After graduating from St. Louis University School of Medicine, he completed his Internal Medicine residency and Rheumatology fellowship at the University of California, Irvine. He is board-certified in Internal Medicine and Rheumatology.

EST (expressed sequence tag): A unique stretch of DNA within a coding region of a gene that is useful for identifying full-length genes and serves as a landmark for mapping. An EST is a sequence tagged site (STS) derived from cDNA.

An STS is a short segment of DNA which occurs but once in the genome and whose location and base sequence are known. STSs are detectable by the polymerase chain reaction (PCR), are helpful in localizing and orienting mapping and sequence data, and serve as landmarks in the physical map of the genome.

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Reviewed on 12/4/2018

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